UniSC findings ‘fast-track treatment’
A Queensland research collaboration has identified a simple way to safely kill coronavirus in patient diagnostic samples by using a unique preparation liquid developed by UniSC.
UniSC researchers say it has the potential to save lives by speeding up testing, leading to faster test results and faster treatment.
The study published in Frontiers in Microbiology involved the University of the Sunshine Coast (UniSC), QIMR Berghofer Medical Research Institute, the University of Queensland and BioCifer, a Queensland based medical biotech company.
UniSC Associate Professor of Molecular Engineering Joanne Macdonald said the liquid reagent had the potential to save lives by speeding up diagnostic testing, leading to faster test results and faster treatment.
“The study showed that the unique composition of the reagent could extract RNA in patient samples in little as five to ten minutes – and importantly, rapidly inactivate SARS-CoV-2 to make testing safer,” she said.
“It’s a simple three-step protocol. No magnetic beads, no spin columns, no elution. Just mix, hold for a short time at room temperature, and your sample is ready for PCR,” said Dr Macdonald, who is also BioCifer’s co-founder and current Chief Technology Officer.
“While many people think that SARS-CoV-2 is ‘old news’, it’s important to realise that it is still the third leading cause of death in Australia after heart disease and dementia, and early detection continues to be critical, particularly in our elderly and vulnerable populations.”
UniSC Associate Professor of Molecular Engineering Joanne Macdonald says liquid reagent – developed by UniSC researchers – has the potential to save lives.
The reagent, known as TNA-Cifer Reagent E, was originally developed at UniSC’s Centre for Bioinnovation. QIMR Berghofer performed the inactivation testing experiments at its SARS-CoV-2 PC3 Biosecurity facility.
The University of Queensland assisted by testing its ability to detect SARS-CoV-2 in patient samples.
Professor Andreas Suhrbier, who leads QIMR Berghofer’s Inflammation Biology Group, said faster, safer and cheaper, yet accurate, diagnosis clearly had many advantages.
“It is very gratifying to see our state-of-the-art biosecurity facility, that we operate thanks to generous philanthropic donations from The Brazil Family Foundation and others, help Queensland biotech produce new products that will transform diagnosis of hazardous pathogens,” he said.
QIMR Berghofer research officer and BioCifer consultant Dr Daniel Rawle said the findings paved the way for diagnosis to be performed in resource poor settings.
The TNA-Cifer reagent has also been demonstrated, in other studies, to potentially enable safe, rapid onsite testing of other diseases such as dengue, Nipah and Hendra viruses.
Dr Macdonald said the reagent has potential to help manage outbreaks of disease by preventing testing backlogs and providing real-time information.